Pioneer Research Anahita

In vitro Tools Advancing life

ADVANCED IN VITRO CELL TECHNOLOGY

Our advanced cell models and cell-based assays are reliable, in vitro systems for substance screening. We use three dimensional systems, because in three dimensions and appropriate microenvironment cells grow better.

PIONEER RESEARCH ANAHITA

Our mission is to protect, and improve human health. Our vision is that our research can contribute to ensure that cancer, as well as other diseases rates will decrease and that all life forms benefit from our achievements.

PERSONALIZED CANCER THERAPY

Our focus relies on cancer research and personalized cancer therapy in Iran due to accelerating cancer rates. Our cell and molecular biology experts use advanced cell culture techniques to establish primary cancer cell cultures of high accuracy for personalized cancer therapy.

Services

We focus on innovative applications to achieve results of highest relevance.

Substance testing

Many new developed substances or the ones that appear in our everyday lives are not tested a priori. They will come into contact with humans, posing risks for human health. Our substance testing service in various human cell culture and tissue models is dedicated mainly to all producers and distributors of food, , chemicals, cosmetic products, drugs, and nano materials, so that they can recommend an in vitro tested, harmless substance to customers. We carry out tests concerning for instance cell viability, cell death, apoptosis, cell proliferation, inflammatory response, cellular redox state and anti-oxidative cell defence, and cell stress. If you need a test that is not listed above, please feel free to contact us.

Uptake of substances/Permeability studies

Cellular barriers like intestine, lung or skin control selective uptake of substances, such as chemicals, drugs, nutrients, xenobiotics, and germs, play critical roles in human health. Barrier function should be verified, because barrier disfunction contributes to disease pathogenesis. For instance, if transport of substances across the intestinal epithelial barrier is inhibited by drugs, the barrier integrity is lost, and the architecture of the intestinal cells is changed. In consequence, this could induce drug resistance in cancer therapy and severe malnutrition.

Cell-based assays

Cell-based assays are any method which is used to assess the cellular response due to compounds or materials such as chemicals, cosmetic products, drugs, food, food supplements, feed, feed supplements, and nano materials. In opposite to biochemical assays the cell-based assays include the response of living cells either directly or indirectly through the cell metabolic pathways. The in vitro assessment using cell culture evaluations is the first step to determine if significant cell response exists for the given compound or material and reduces subsequent effort to predict their performances in vivo.

Training

The training concept is based on the teaching of practical and theoretical cell and molecular biology knowledge in workshop and courses. Training in Anahita academy and training centre is carried out in small groups, depending on level of experience, enabling a targeted and efficient training. Our customers get efficient training on different topics and courses from highly skilled lecturers. In addition, we offer competent and friendly service. We will introduce you into fascinating cell and molecular biological topics or, if you are on an experienced level, we will keep you up-to date.

DNA, RNA and Protein analysis

On the molecular level we specialize in DNA, RNA, and protein analysis from different cells and various tissues. We can provide you further services such as primer design, PCR, real time PCR, Western blot analysis, and other molecular biological analysis. In case of special questions you are welcome to contact our specialists for further analysis.

Research

Personalized Tumor therapy

Cancer cases increase due to the rising life expectancy, environmental influences, such as air pollution and stress and some other factors. Despite intensive research and recent progress in cancer therapies it is difficult to predict the response of patients to targeted treatments because of interpatient (tumor genotype variations between patients) and intratumour heterogeneity (subpopulations of cancer cells with unique genomes and behaviour in the same patient). Low responsiveness or in the worst case resistance against a certain chemotherapy occurs frequently.
Personalized cancer therapy is a treatment strategy for predicting which patients tend to react to certain treatments and leads to more effective diagnosis and/or cancer therapies. This therapy concept is based on the fact, that each cancer is unique. Therefore, different tumor biomarkers and differences in drug metabolism, drug response and drug toxicity are associated with patient prognosis and tumor response to therapy.
We aim to provide personalized cancer screening to determine the optimum of individualized therapy and improve health care of patients.
Living cancer tissues or cancer cells from a surgical specimen originated from patients are cultured in vitro. The tumor cells are subsequently treated with a spectrum of anti-cancer drugs to determine which drug or drug cocktail is most effective. In vitro tumor cell proliferation, tumor growth, cell death, metabolic activity are monitored over time. The results can usually be provided to patients and their oncologists whithin a short period of time, before the initiation or adaptation of therapy.

Cancer cells

Tumors are functionally heterogeneous and hierarchical. The Cancer Stem Cell Hypothesis implies that tumors are composed of a small number of tumor initiating cells called cancer stem cells and cells that arise from cancer stem cells but cannot initiate tumors. The concept of cancer stem cells has significant clinical implications because cancer stem cells may have different sensitivities to radiation or chemotherapy. Cure of cancer may require elimination of the minority cancer stem cell population of the tumor as well as the non cancer stem cell majority of cancer cells. If some cancer stem cells appear to be more resistant to therapeutical treatment than other cells of the tumor, cancer elimination is not complete and tumor will return after a while. To predict human cancer responses the in vivo growth environment has to be resembled closely. Therefore, we establish cancer cell cultures from primary material from patients that grow better in appropriate microenvironments under defined culture conditions.

In addition, we use immortal cancer cell lines for cancer research.

BioAFM in Cancer Diagnostics

Atomic force microscopy (AFM) analysis is a non-invasive imaging method to characterize and discriminate cancerous cells from healthy cells. This is a national project in collaboration with Ara Research Company, Teheran, Iran.

PhotoCORMs

PhotoCORMs are photoactive carbon monoxide (CO)-Releasing Molecules which could have different physiological effects in human body. In an in vitro model, anti-apoptotic and anti-inflammatory effects of new PhotoCORMs complexes including water-soluble derivatives will be tested. This is an international project together with Univ.-Prof. DI Dr. Guenther Knoer, Institute for inorganic chemistry - Center for Nanobionics and Photochemical Sciences (CNPS), Johannes Kepler University Linz, Austria.

Products

Schuettbiotec GmbH

Products for Biotechnology, Microbiology, Life Sciences: Anaerobic jars, The new sense of flaming, Safety Bunsen Burner, Glasses, Beakers, Bottles & Caps, Ice-buckets, Manual Colony Counter, Schuett Colony Quant (rapid and reliable counting), Containers for sterilization and storage, Cuvettes (Vessel Rinser and Storage), EggPunch (Pneumatic Egg-Opener), Helpful gadgets for your Laboratory, Homogenisation with Perfection, INCUDRIVE Rollersystems and Incubators, Schuett Solaris (sterilize with light), Yeast Genetics (TDM Tetrad Dissection Microscope Ci-L), Schuett Petriturn (Turntables for Petri dishes & Inoculating hooks), EcoVac safety-vacuum-system (Removal of liquid excess), Multi-Pulse-Vortexer (Mixing and Homogenizing), RULLA 2 (Wet-Penetration-Test)

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FibreCell Systems

Hollow fiber bioreactors and relate products designed for the following applications: Monoclonal Antibody Production, Recombinant Protein Production, Endothelial Cell Culture, Lymphocyte Culture and Expansion, In Vitro Toxicology

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Boxer Pumps

Diaphragm Pumps for Gases, Diaphragm Pumps for Liquids, Peristaltic Pumps for Liquids

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HYUNDAI MICRO

Plastic Ware, Syringe Filter, Membrane Filter, Filter Paper, Column Kit

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Application of our Products

Barrier models: Cell barriers are polar organized cell layers between different compartments that protect the body and control the selective uptake of substances. For instance, epithelial cell barriers are located in the intestine, lung and skin. Active and passive transport of molecules and ions occur from the apical to the basolateral side and vice versa. This requires passage either through the cells (transcellular route) or between the cells and thus through junctional complexes (paracellular route).

Barrier models

Bioreactors: A bioreactor is a device for growing organisms such as algae, bacteria, yeast, animal or human cells that are used in the biotechnological production of substances such as pharmaceuticals, antibodies, or vaccines, or for the bioconversion of organic waste. Bioreactors are used for different purposes: Monoclonal Antibodies, Recombinant Proteins, In vitro toxicology, Endothelial Cell Culture, Exosomes, Conditioned Medium, Virus, Tissue engineering

Bioreactors

Tissue Models

Skin

The three-dimensional human skin models are used for the skin irritation and skin corrosion test and comprise in the simplest case a reconstructed epidermis with a functional stratum corneum. We address companies that wish to comply with the provisions of the European Union chemicals regulation REACH and the European Union directive on the protection of animals used for experimental and scientific purposes. In addition, this is essential for the cosmetically industry. As from March 2009 in the European Union starting materials for cosmetics may no longer be tested on animals (7th Amendment to the EU Cosmetics Directive).

Lung

Lung cancer is a common cause of cancer-related death worldwide and effective treatments for patients with lung cancer are still missing. One reason for the lack of progress in lung cancer research is the lack of an accurate model that resembles the biological processes that occur in patients with lung cancer. To construct in vitro models of human lung tissue, tissue-specific epithelial cells are co-cultured with other cells in a 3D matrix. In contrast to other barrier models, the top is exposed to air. The lung tissue model provides a physiologically relevant tissue microenvironment for both basic and applied research and compound studies.

Intestine

The intestine is the most important part of the digestive tract and is, in adult humans about eight meters long and has a surface area of ​​about 400 to 500 m². Intestinal cells are specialized for the selective digestion and absorption of substances after eating and drinking. The single-intestinal epithelium limits the intestinal lumen from the side where the substances are being released into the bloodstream and forms a natural barrier. Maintaining the intestinal barrier is vital. Nutrients should pass the intestinal barrier unhindered, while in contrast, passage of germs and toxins should be blocked. The active ingredients are absorbed by the intestinal lumen through the intestinal epithelium by various transport mechanisms. Many processes are involved in the regulation of the transport events and diseases arise when the intestinal barrier function is acutely or chronically damaged or even destroyed.

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